The development of pharmacotherapies that promote remyelination is a high priority for multiple sclerosis (MS), due to their potential for neuroprotection and restoration of function through repair of demyelinated lesions. A novel preparation of gold nanocrystals demonstrated robust remyelinating activity in response to demyelinating. The serum levels of NOS and CYP27B1enzyme for 90 MS patients and 30 controls were measured using enzyme-linked immune sorbent assay (ELISA). This project includes the green synthesis of gold nanoparticles using cardamom husks and a chemical reduction method. These methods resulted in pure spherical nanoparticles with small diameters of up to 20 nm without agglomerations. The nano-synthesized were investigated using X-ray diffraction (XRD), Photoluminescence (PL) and TEM. The toxicological effect of cardamom husks with gold nanoparticles AuNPs and AuNPs by chemical reduction on the growth of human neuroblastoma cell line (SH-SY5Y) cells was studied by examining the toxicity test (MTT) for 48 hours of exposure. The results showed that cardamom husks with gold nanoparticles AuNPs have the ability to inhibit the growth of SH-SY5Y cancer neuron cell lines, which proved effective at a concentration of 200 μmol/mL, as it achieved a killing rate of SH-SY5Y neurons at 46.644 % compared to the control group, which is the fibroblast cell line normal skin derived from human stem cells (HDFn), the toxicity rate was 19.637% for the presence of gold nanoparticles prepared using cardamom husks, while the toxicity for HDFn cells was 49.691% in the presence of gold nanoparticles prepared by the chemical method, which gives a clear inference that the method used for the synthesis of gold using cardamom husks achieved toxicity of 2.4 times less, which is considered a promising method for medical use. The work aimed to study the effects of nanoparticles on the levels of NOS and CYP27B1activity in the serum of newly diagnosed MS patients. The current study of AuNPs could be a stepping stone for future work to improve the binding between the vitamin D and its receptors inside the body and could be used to improve and modify a competitive ELISA method. These effects were the nanoparticles have an inhibition effect on the NOS synthase levels in sera of N-RRMS with MS patients. While the nanoparticles have not demonstrated effects inhibition on the binding between anti- CYP27B1antibody and antigen CYP27B1level in pool sera of MS patients.