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Antibiofilm Activity of Eugenol against Aggregatibacter Actinomycetemcomitans ATCC 43718 (serotype B)

Article

Last updated: 01 Jan 2025

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Tags

Pharmaceutical Chemistry

Abstract

Eugenol is the major constituent [70% to 90%] in the aromatic oil extract from Syzygium aromaticum (cloves) and Cinnamomum zeylanicuma, which is widely used as a flavouring for meats, stews, cakes, and teas. This plays a major role in the microbial activity against oral bacteria, specifically Aggregatibacter actinomycetemcomitans ATCC 43718 (serotype B), which causes periodontitis in humans. Therefore, this study aims to evaluate eugenol for its antibiofilm activity against Aggregatibacter actinomycetemcomitans ATCC 43718 (serotype B). An inhibition growth assay was used with the microdilution method, which often utilized a 96-microplate with an anaerobic technique. Absorbance was also measured by a microplate reader on λ of 595 nm, with the optical density (OD) value used to determine the inhibition percentage. To evaluate biofilm bacterial growth after the treatment, SEM analysis was adopted. The results showed that eugenol had an inhibitory effect on A. actinomycetemcomitans at 0.125 % v/v, according to the MBIC50 value. Eugenol showed significant antibiofilm activity (P < 0.05) against A. Actinomycetemcomitans. This proved that the biofilm bacterial growth was effectively inhibited by the chemical compound. In this case, the extracellular polymeric substance (EPS) matrix degraded after the treatment of the cell biofilm. Based on these results, eugenol was observed to have a great potential in periodontitis, as an inhibitor against the biofilm growth of A. actinomycetemcomitans ATCC 43718 (serotype B).

DOI

10.21608/ejchem.2022.143899.6278

Keywords

Biofilm, eugenol, Aggregatibacter actinomycetemcomitans ATCC 43718 (serotype B)

Authors

First Name

Diyah Tri

Last Name

Utami

MiddleName

-

Affiliation

Department of Pharmacy, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Surakarta, 57126, Indonesia, Postdoctoral Program Pusat Riset Bahan Baku Obat dan Obat Tradisional (Research center for Medical Raw Materials and Traditional Medicine), National Research &amp; Innovation Agency, Kawasan Puspiptek, Serpong 15314, Indonesia

Email

diyahtriutami12@staff.uns.ac.id

City

-

Orcid

-

First Name

Triana

Last Name

Hertiani

MiddleName

-

Affiliation

Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia. , Pusat Kolaborasi Riset Biofilm (Biofilm Research Collaboration Centre, UGM-BRIN), Jl. Farmako Sekip Utara, Yogyakarta 55281, Indonesia

Email

triana_hertiani@ugm.ac.id

City

-

Orcid

-

First Name

Sylvia Utami Tunjung

Last Name

Pratiwi

MiddleName

-

Affiliation

Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia. , Pusat Kolaborasi Riset Biofilm (Biofilm Research Collaboration Centre, UGM-BRIN), Jl. Farmako Sekip Utara, Yogyakarta 55281, Indonesia

Email

sylvia_pratiwi@ugm.ac.id

City

-

Orcid

-

First Name

Ahmad

Last Name

Randy

MiddleName

-

Affiliation

Pusat Riset Bahan Baku Obat dan Obat Tradisional (Research center for Medical Raw Materials and Traditional Medicine), National Research & Innovation Agency, Kawasan Puspiptek, Serpong 15314, Indonesia

Email

ahmad.randy@brin.go.id

City

-

Orcid

-

First Name

Anif Nur

Last Name

Artanti

MiddleName

-

Affiliation

Department of Pharmacy, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Surakarta, 57126, Indonesia

Email

anif.apt@staff.uns.ac.id

City

-

Orcid

-

First Name

Dian Eka

Last Name

Ermawati

MiddleName

-

Affiliation

Department of Pharmacy, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Surakarta, 57126, Indonesia

Email

dianekae@staff.uns.ac.id

City

-

Orcid

-

First Name

Sholicah

Last Name

Rahmani

MiddleName

-

Affiliation

Department of Pharmacy, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Surakarta, 57126, Indonesia

Email

licha@staff.uns.ac.id

City

-

Orcid

-

First Name

Heru

Last Name

Sasongko

MiddleName

-

Affiliation

Department of Pharmacy, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Surakarta, 57126, Indonesia

Email

heru_sasongko@staff.uns.ac.id

City

-

Orcid

-

First Name

Wisnu

Last Name

Kundarto

MiddleName

-

Affiliation

Department of Pharmacy, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Surakarta, 57126, Indonesia

Email

wisnukundarto@staff.uns.ac.id

City

-

Orcid

-

First Name

M Fiqri

Last Name

Zulpadly

MiddleName

-

Affiliation

Department of Pharmacy, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Surakarta, 57126, Indonesia

Email

zulpadlyf@staff.uns.ac.id

City

-

Orcid

-

Volume

66

Article Issue

4

Related Issue

40820

Issue Date

2023-04-01

Receive Date

2022-06-10

Publish Date

2023-04-01

Page Start

205

Page End

208

Print ISSN

0449-2285

Online ISSN

2357-0245

Article File

EJCHEM_Volume 66_Issue 4_Pages 205-208.pdf

PDF . 310.8kB

Link

https://ejchem.journals.ekb.eg/article_252611.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=252611

Order

252,611

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Antibiofilm Activity of Eugenol against Aggregatibacter Actinomycetemcomitans ATCC 43718 (serotype B)

Details

Type

Article

Created At

30 Dec 2024