Tilapia fish species have been identified as excellent source of inexpensive protein for the nutritional benefit of human beings. This study was aimed to evaluate the nutritive value and healthy safe quality of some commercial fish species (Oreochromis niloticus, Sarotherodon galilaeus and Coptodon zillii) collected from some River Nile Islands (El-Qeratten, El-Waraq, El-Zamalek, and El-Manial) during July 2020. The results showed that, the mean values of moisture, protein, lipid and ash of O. niloticus, S. galilaeus and C. zillii collected from four River Nile Island during July 2020 were (79.25, 18.28, 1.48, 0.73%); (79.46, 18.23, 1.37, 0.71%) and (78.56, 19.34, 1.12, 0.43%), respectively. On the other hand, the pH, TVBN, TMA and TBA values were ranged 6.01-6.18; 14.11-15.80 mg/100g; 1.01-1.22 mg/100g and 0.49-0.40 mg MDA/kg, respectively. Referring to healthy safe quality aspects of some commercial fish species collected from some River Nile Islands during July 2020 it could be concluded that, the analyzed fish samples didn't contained any Salmonella or Staphylococcus aureus bacteria, while total bacterial count and coliform bacteria were ranged 3.18-3.66 and 2.01-2.249 (log10 cfu/g ), respectively. The presents study shows that, the Sb, As, Cd, Se, Sn and V were below the detection limits. Whereas, the mean values of Al, Cr, Co, Cu, Fe, Pb, Mn, Ni and Zn of O. niloticus were 20.59, 3.06, 0.09, 4.54, 25.10, 0.21, 1.86, 1.53, 22.08 mg/kg ww, respectively, while it were ranged 19.01, 3.06, 0.10, 4.47, 20.90, 0.33, 1.87, 1.75, 20.96 mg/kg ww, respectively for S. galilaeus and it were recorded 18.87, 3.12, 0.10, 5.48, 21.38, 0.29, 1.85, 1.98, 23.26 mg/kg ww, respectively for C. zillii. Also, the results indicated that, heavy metals concentrations in collected fish samples didn't exceed the maximum permissible limit set by WHO/FAO (2011). Based on the obtained results it could be concluded that fish samples collected from River Nile Island during July 2020 were suitable for human consumption and didn't pose any hazards to human health.