This work is concerned with the studies of the bioactivities of the prepared Cu-MOF compound and its modification with diatomite. This work aims to preparation and characterization of Cu-MOF nano-particles and evaluation against some organisms. Otherwise, the structure of the Cu-MOF was modified and the effects of modification on the bioactivity were investigated. Cu-MOF/diatomite was prepared and their characterizations by using different methods were carried out. The characterization by flourier transforms Infrared red (FTIR), X-ray diffraction (XRD), transmission electron microscope (TEM) and thermal degradation analysis (TGA) indicates that Cu-MOF incorporated onto the diatomite unit. The thermogravimetric studies, also showed that as the diatomite particles coated with the organic fragments prevent their thermal decomposition at low temperature. The bioactivity of Cu-MOF/diatomite was studied by using the agar diffusion technique; it is found that incorporated Cu-MOF onto diatomite increases the bioactivity of Cu-MOF.