1.1. Medicinal plants (MP) have recognized to be a rich source of biologically active compounds for the development of new lead chemicals for pharmaceuticals. Screening of Pandanus tectorius and Rosmarinus offcinalis plant extracts confirms the presence of various phytochemicals like carbohydrates, cardiac glycosides, coumarin, flavonoids, tannins, terpenoid, steroids and phenols in the selected plants. While other phytochemicals as anthraquinone, alkaloids, saponins, proteins and phytosterol were detected and varied based on the plant constituents. The antibacterial, antifungal and antioxidant activities of the tow plant extracts were established. The antioxidant activity was evaluated by DPPH. The anti-microbial activity determined by the agar well-diffusion method. The results confirm the role of these extracts as promising potent antioxidants and moderate anti-microbial agents. The objective of this study was to determine the phytochemical screening, analysis by LC/MS/MS, the antioxidant, antimicrobial activities of Pandanus tectorius and Rosmarinus offcinalis ethanolec extract that obtained from AlBaha in Kingdom of Saudi Arabia.