This study aimed to investigate in vitro the effect of Curcumin and Vitamin E by using liposome as a drug-delivery system and evaluate the cytotoxic potential of these liposome using laryngeal carcinoma Hep-2 cell line. Curcumin and Vitamin E liposomes were characterized in terms of size distribution, ζ-potential, Fourier transform infrared (FT-IR) spectroscopy and Differential scanning calorimetry (DSC). In the cell count assay, the viability of cells decreased as the concentration of the compounds increased. The cell viability declined to about 4.5% at a concentration of 400 µM for Curcumin and Curcumin-loaded liposomes, while declined to about 52.1% and 53.6 % at a concentration of 400 µM for Vitamin E and Vitamin E loaded liposomes respectively. Finally, this result indicates that Curcumin and its liposomal form have strong inhibition of cell growth and continually increasing inhibition with increased concentrations against Hep-2 cell line. vitamin E showed limited effect on Hep-2 cancer cells, while Curcumin combined with Vitamin E or its liposomal forms indicated a significant reduction in the cell viability when tested at the same concentration (400 µg/ml).