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249733

Simple Green Spectrophotometric & Chromatographic Assay of the Oral Antiviral Treatment of COVID -19: Molnupiravir -EIDD-2801

Article

Last updated: 01 Jan 2025

Subjects

-

Tags

Analytical chemistry

Abstract

Although vaccination for “Coronavirus disease 2019" is currently available, effective antiviral therapy is of great importance. The presence of easily administrated oral antivirals was a goal since beginning of the pandemic. The end of 2021 witnessed the emergency use authorization to Merck oral antiviral drug Molnupiravir (MOL). This study presents three analytical procedures to assay MOL in its raw material and dosage form. Method I: direct spectrophotometric measuring at λmax 233 nm using methanol as a solvent. Method II: HPTLC method, using methanol and glacial acetic acid as mobile phase followed by densitometric scanning of MOL bands at its λmax. Method III: RP-HPLC-DAD procedure, where MOL is separated only in 5 minutes, using isocratic elution of acetonitrile and distilled water acidified with orthophosphoric acid (pH 3) with ratio 87:13 (flow rate 1 mL/min.). The DAD detection was done also at 233 nm. These methods were validated to be ready for MOL rapid quality control assay in its fast massive production with good linearity correlation coefficients in ranges of 2.5-20 μg/mL, 0.03-0.38 μg/band and 0.025-10 μg/mL, for methods I, II & III, respectively. Limits of detections of 0.53 μg/mL, 0.01 μg/band & 0.005 μg/mL of methods I, II & III, respectively show the proposed methods' sensitivity. In addition, the three methods were applied for assaying MOL in laboratory prepared capsules to prove the methods' selectivity. Finally, the greenness of the three proposed methods was assessed & compared to those of the previously reported methods for MOL single assay by AGREE metric for greenness assessment.

DOI

10.21608/ejchem.2022.135659.5976

Keywords

Molnupiravir, COVID-19, Spectrophotometry, HPTLC, RP-HPLC, validation, Green

Authors

First Name

mona

Last Name

Abdel Moneim

MiddleName

-

Affiliation

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Pharos University in Alexandria, Alexandria, Egypt.

Email

mona.abdelmoneim@pua.edu.eg

City

-

Orcid

-

First Name

Miranda

Last Name

Kamal

MiddleName

-

Affiliation

Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Damanhour University, Beheira, Egypt.

Email

mirandafawzy@yahoo.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Hamdy

MiddleName

-

Affiliation

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Pharos University in Alexandria, Alexandria, Egypt.

Email

mohamed.hamdy@pua.edu.eg

City

-

Orcid

-

Volume

66

Article Issue

3

Related Issue

40217

Issue Date

2023-03-01

Receive Date

2022-04-24

Publish Date

2023-03-01

Page Start

125

Page End

131

Print ISSN

0449-2285

Online ISSN

2357-0245

Link

https://ejchem.journals.ekb.eg/article_249733.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=249733

Order

249,733

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Simple Green Spectrophotometric & Chromatographic Assay of the Oral Antiviral Treatment of COVID -19: Molnupiravir -EIDD-2801

Details

Type

Article

Created At

30 Dec 2024