Silver nanoparticles (AgNPs) and gold nanosheets (Au nanosheets) have outstanding properties that make them suitable for use in multiple fields including the biomedical field. In the current study, silver nanoparticles (AgNPs) and gold nanosheets (Au nanosheets) were fabricated by chemical method and their mixtures using deposition technique, which is one of the most popular methods. The production of these materials is simple because they produce molecules with high bioavailability and low toxicity. Nanostructures (Au, Ag, and Au+Ag nanosheets) have been used to treat a variety of cancers, including colorectal cancer, due to their biocompatibility, large surface area, and high dispersibility. Several analysis methods (X-ray diffraction, optical properties, scanning electron microscopy, transmission electron microscopy, etc.) have been used to study the characterization of the synthesized nanomaterials. Moreover, these nanomaterials have been used in cancer treatment and antibacterial activities against various bacteria. We present here a technological platform for engineering Au nanotopographies by templated electrodeposition on antibacterial surfaces. Three different types of nanostructures were fabricated: nanoparticles, nanorods and nanosheets. The nanoparticles are the basic structures and are 50 nm in diameter and 100 nm in height. Particular arrangement of the nanoparticles in various geometries formed nanorods and nanosheets. Flat surfaces, rough substrate surfaces, and various nanostructured surfaces were compared for their abilities to attach and kill bacterial cells. Methicillin-resistant Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli and Kiebsiella Sp. a Gram-positive and a Gram-negative bacterial strain responsible for many infections in health care system, was used as the model bacterial strain. It was found that all the Au nanostructures, regardless their shapes, exhibited similar excellent antibacterial properties. A comparison of live cells attached to nanotopographic surfaces showed that the number of live Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli and Kiebsiella Sp. cells was <1% of that from flat and rough reference surfaces. Our micro/nanofabrication process is a scalable approach based on cost-efficient self-organization and provides potential for further developing functional surfaces to study the behavior of microbes on nanoscale topographies. According to the study, these nanoparticles kill more than 80% of cancer cell lines. A comparison was made between the goods.