In this work new heterocyclic derivatives were prepared. The most available (Sulfadiazine) compound treated with different aromatic aldehydes to synthesis (F1-F5) imine derivatives then cycloaddition reaction of imines with phenylthioacetic acid to produced azetidin-2-one derivatives(F6,F7). compound F1 treated ethylene glycol and hydrazine to get (F8) . (F9,F10) compounds has been synthesis by reacting (F1) compound with maleic anhydride and phthalic anhydride in dry benzene . compound F8 treated p-hydroxy benzaldehyde to get (F11) compound. Reaction (F11) with benzoyl chloride gave (F12) compound. Also, (F12) compound treated with urea to get urease derivative .These synthesized compounds (F1-F13) evaluated for their antimicrobial activity and antioxidant activity.