Pot experiments were performed throughout two winter seasons of (2019/2020) and (2020/2021) at the greenhouse of National Research Centre, Giza, Egypt. The experiment was performed to evaluate the bio-herbicidal potentiality of aqueous extract of root and leaf of red radish at 1.5%, 3.0%, 4.5% and compare them to mixed two chemical herbicides (Topik 240 EC (Clodinafop-Propargyl) and broad weed Basagran (Bentazon 48 EC)) at recommended doses 140 g fed-1 and 1 L fed-1, respectively) in controlling weeds (Phalaris minor and Malva parviflora) associated with lupine plants. Results show that the dry weight of the two weeds under investigation was significantly reduced by all applied aqueous extracts (either radish root or leaf). Leaf aqueous extract was more effective than root aqueous extract in controlling weeds. It is worthy to mention that the two mixed applied herbicides gave complete eradication of both weeds. In addition, all applied aqueous extracts significantly increased growth, photosynthetic pigments, seed yield and yield components of lupine plants. The inhibitory effect of radish aqueous extracts on weeds or its stimulatory effect on lupine plants was increased by increasing concentration of the extracts. The leaf aqueous extract and root aqueous extract at 4.5% were the most optimum treatments in increasing growth and seed yield of lupine accompanied by the highest decrease in weed dry weight relative to unweeded treatment. The results suggested using the aqueous extracts of radish leaf and root especially at highest concentration (4.5%) in controlling weeds associated with lupine plants.