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271104

Green Bio Estimation of Co-Administered Non-Steroidal Anti Inflammatory Drug Diclofenac and Leflunomide in Rat Plasma by New HPLC Method

Article

Last updated: 01 Jan 2025

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Abstract

An eco-friendly HPLC- DAD method is introduced for the bioanalysis of co-administered non-steroidal anti-inflammatory drug diclofenac and leflunomide in rat plasma. Leflunomide and NSAIDs are commonly prescribed in combination This method aimed to estimate the co-adminstered leflunomide and diclofenac combination for their simultaneous separation and quantification The method was conducted with gradient elution of a mobile phase composed of ethanol and phosphate buffer with a flow rate of 1 mL/min over a Zorbax Eclipse Plus-C18 (4.6 × 250 mm × 5 µm) column. The drug peaks were picked using a diode array detector at 275 nm. The developed method was validated according to ICH guidelines in terms of accuracy, precision, linearity, range, LOD and LOQ. The concentration range was found to be linear in the range of 2 – 60 µg/mL. The LOD and LOQ values were found to be very small (0.16, 0.47 μg/mL and 0.22, 0.68 μg/mL) for Diclofenac and Leflunommide respectively. The % RSD and the % R were found within the acceptable range. The method was applied for in vitro and in vivo analysis of diclofenac and leflunomide with mean recoveries of 97.5 to 102.30 and 97.31 to 101.20 respectively.

DOI

10.21608/ejchem.2022.156644.6786

Keywords

Green method, Bioanalysis, NSAID, leflunomide

Authors

First Name

Ossama

Last Name

Abdullatef

MiddleName

-

Affiliation

Faculty of Pharmacy, Pharmaceutical Chemistry Department, Pharos University, Smouha, Alexandria, Egypt

Email

ossama_abdullatef@yahoo.com

City

alexandria

Orcid

-

First Name

Yasmine

Last Name

Khalifa

MiddleName

-

Affiliation

Faculty of Pharmacy, Pharmaceutical Chemistry Department, Pharos University, Smouha, Alexandria, Egypt

Email

yasmine.khalifa@pua.edu.eg

City

-

Orcid

-

First Name

Samir

Last Name

Morshedy

MiddleName

-

Affiliation

Faculty of Pharmacy, Pharmaceutical Analytical Chemistry Department, Damnhour University, Damnhour, Egypt

Email

dr.samirmorshedy@pharm.dmu.edu.eg

City

-

Orcid

0000-0002-6362-5118

First Name

Wael

Last Name

Talaat

MiddleName

-

Affiliation

Faculty of Pharmacy, Pharmaceutical Analytical Chemistry Department, Damnhour University, Damnhour, Egypt

Email

wtalaat2017@gmail.com

City

-

Orcid

-

Volume

65

Article Issue

132

Related Issue

39238

Issue Date

2022-12-01

Receive Date

2022-08-16

Publish Date

2022-12-01

Page Start

1,307

Page End

1,316

Print ISSN

0449-2285

Online ISSN

2357-0245

Link

https://ejchem.journals.ekb.eg/article_271104.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=271104

Order

271,104

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Green Bio Estimation of Co-Administered Non-Steroidal Anti Inflammatory Drug Diclofenac and Leflunomide in Rat Plasma by New HPLC Method

Details

Type

Article

Created At

30 Dec 2024