The current study used a green technique that involves an aqueous leaf extract of Morus alba (mulberry) to synthesize silver nanoparticles (AgNPs) and assess their antibacterial and antiviral capabilities. The optical studies revealed a color shift from colorless - light yellowish to dark yellowish-brown as an indication of the reduction of silver metal ions & creation of silver nanoparticles and Ultraviolet-visible spectroscopy (UV-Vis) examination revealed a surface plasmon resonance (SPR) peak at 430 nm, indicating nano-silver (AgNPs) production. In addition, Fourier-transform infrared spectroscopy (FTIR) studies indicated that proteins, carbohydrates, and secondary metabolites may act as reducing and capping agents. While the Transmission electron microscopy (TEM) examination, revealed the produced nanoparticles were spherical, oval, and triangular with particle sizes ranging from 20 to 100 nm. Moreover, X-ray diffraction (XRD) measurements, show the crystalline structure of green synthesized (AgNPs) that are preferentially orientated along (1 1 1), (2 0 0), (220), and (311) planes. Furthermore, Silver nanoparticles had antibacterial action against both Gram-positive and Gram-negative bacteria, with the best results against Staphylococcus aureus ATCC 6538 at the highest concentration (conc.) of biosynthesized silver nanoparticles (400 µg/ml) with a maximum diameter of inhibition activity (24.33 mm), whereas the Minimal Inhibitory Concentration (MIC) was recorded at conc. (50 µg/ml) against both gram-positive and gram-negative bacteria, with the highest action against gram-negative bacteria Pseudomonas aeruginosa with a maximum diameter of inhibition activity (13.56 mm). In addition, silver nanoparticles showed promising antiviral activity against both Hepatitis B virus (HBV) and Hepatitis D virus (HDV) with maximum inhibition percent of 94.64 and 100 at conc. of 15.63 µg/ml of AgNPs for each virus respectively, and with minimal cytotoxic concentration (conc.) up to 99.5 percent and 90.66 percent viability on Human derived hepatoma (HepG2) cell line treated with silver nanoparticles at conc. of 7.81 µg/ml and 15.6 µg/ml respectively.