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244911

Bioengineering of Zinc Oxide Nanoparticles As Therapeutics for Immunomodulatory and Antimicrobial Activities

Article

Last updated: 01 Jan 2025

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Subjects

-

Tags

Nanotechnology

Abstract

In the present work, eco-friendly biosynthesis of zinc oxide nanoparticles (ZnONPs) and their immunostimulatory and antimicrobial activity were investigated. Streptomyces rochei, the ZnONPs manufacturer, were isolated and identified. The morphology and characterization of ZnONPs using UV-Vis, DLS, TEM, and XRD, were investigated. The results showed that the spherical shape of ZnONPs was with a mean diameter of about 36nm. The viability of the HFB-4 and Vero cells was decreased to 42 and 46% respectively after treatment with a dose of 16µl/ml of ZnONPs. ZnONPs showed a significant immunostimulatory effect with 137.2 ± 0.18% and increased CD4+ and CD8+ lymphocytes level with 85.2 ± 0.07% and 25.3 ± 0.03%, respectively. Also, they exhibited an antimicrobial activity against both Gram-negative, positive bacteria and fungi with inhibition zone ranging from 16.0 to 28.0 mm and MIC ranging from 8.67 to 125.0 µg/ml. This study highlights that ZnONPs strongly stimulate immune system and have antimicrobial activities.

DOI

10.21608/ejchem.2022.136366.6008

Keywords

Streptomyces rochei, Biosynthesis ZnONPs, Cytotoxicity, immunomodulatory, antimicrobial activity

Authors

First Name

Gamal

Last Name

El-Sherebiny

MiddleName

Mohamed

Affiliation

Prof. of medical microbiology,Botany and Microbiology Department, Faculty Science Al-Azhar University

Email

gamalelsherbiny1970@yahoo.com

City

Cairo

Orcid

0000-0003-3968-0536

First Name

Amir R. Ali

Last Name

Ali

MiddleName

R.

Affiliation

2Applied-Science & Robotics Laboratory for Applied-Mechatronics (ARAtronics Lab.); Mechatronics Engineering Department, German University in Cairo, New Cairo 11835, Egypt.

Email

gamalelsherbiny1970@azhar.edu.eg

City

Cairo

Orcid

-

First Name

Diaa

Last Name

Ali

MiddleName

Mohamed

Affiliation

Department of Botany and Microbiology, Faculty of Science, Al-Azhar University,11884 Nasr City, Cairo, Egypt

Email

diaa271@yahoo.com

City

Cairo

Orcid

-

First Name

Ahamed

Last Name

El-Hawary

MiddleName

S.

Affiliation

Ph.D. Affiliation: Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 11884, Egypt.

Email

ahmad.hawary@azhar.edu.eg

City

Cairo

Orcid

-

First Name

Ahamed

Last Name

Asker

MiddleName

A

Affiliation

Ph.D. Affiliation: Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 11884, Egypt.

Email

drahmed_askar@azhar.edu.eg

City

Cairo

Orcid

-

Volume

65

Article Issue

131

Related Issue

37459

Issue Date

2022-12-01

Receive Date

2022-04-28

Publish Date

2022-12-01

Page Start

1,473

Page End

1,486

Print ISSN

0449-2285

Online ISSN

2357-0245

Article File

EJCHEM_Volume 65_Issue 131_Pages 1473-1486.pdf

PDF . 1.1MB

Link

https://ejchem.journals.ekb.eg/article_244911.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=244911

Order

244,911

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Bioengineering of Zinc Oxide Nanoparticles As Therapeutics for Immunomodulatory and Antimicrobial Activities

Details

Type

Article

Created At

30 Dec 2024