A simple, sensitive, and accurate chromatographic (RP-HPLC) method for simultaneous estimation of norfloxacin, levofloxacin, and moxifloxacin in their combined. Pharmaceutical dosage forms or individually. The HPLC separation was achieved on a Hypersil (C18, (150 mm x 4.6 mm,5 μm particle size) analytical column or equivalent. A Mixture of triethanolamine 1%: acetonitrile 80 % was used as the suitable mobile phase, at a flow rate of 1.2 mL/min and detector wavelength at 280 nm at ambient temperature (25 °C). In the HPLC method, the retention time of norfloxacin, levofloxacin, and moxifloxacin was found to be 1.854, 2.480, and 4.688 min, respectively, and the linearity of the method was obtained in the range from 25 to 80 µg/mL for norfloxacin, levofloxacin, and moxifloxacin. The Correlation coefficient for the method was greater than 0.999 RP-HPLC method validation parameter lay within its acceptance criteria as per the ICHq2 (R1) guideline Validation of analytical procedures. Hence it can be successfully used for routine analysis of norfloxacin, levofloxacin, and moxifloxacin in raw material or pharmaceutical dosage forms.