The textile industry is the second largest industry in Egypt and is important in the economy of the world. Medical textiles are the largest and fastest growing in the industry of textiles. Many compounds used in this field and new coloured compounds applied in the colouration of wool textile and as anti-microbial agents. The compounds with pyrimidine systems have different biological properties, pharmaceutical applications, medical and coloured materials used in different textile materials. The effect of thiopirido-pyrimidine derivatives on the colouring of wool and used as antimicrobials was examined. The colour fastness of the coloured wool fabrics and resistance to washing, Scanning Electron Microscopy (SEM) and radar chart diagram were also determined. The coloured wool has obtained as a good antimicrobial activity against (Staphylococcus aureus), (Escherichia coli), (Candida albicans) and Musor miehei compared to uncolored wool. The impact of the new colour material used as a finishing agent on both the shrinkage % and the recovery by moisture was investigated. The effects of the new coloured properties on wool fabrics and certain functional properties were identified and characterized. The products used as effective antimicrobial agents and will application in future works for textiles.