Background: Improving knee stability and function is known to be possible with anterior cruciate ligament (ACL) reconstruction. Aim of the work: The aim of this study is functionally effective anterior cruciate ligament reconstruction either by peroneus longus autograft or hamstring tendon autograft. Methods: The study was conducted in Orthopedics department, Zagazig University Hospitals, Zagazig, Egypt. 18 Patients were included in our study divided into two groups; Group A (9 ): had ACL reconstruction by peroneus longus graft.Group B (9): had ACL reconstruction by hamstring graft. Results: There is significant improvement in Modified Cincinnati knee rating system, Lysholm and IKDC score Six months Postoperative in both groups. There are no significant changes in Foot and ankle disability index and Ankle hindfoot score in both groups. Conclusion: Peroneus longus tendon autograft is an appropriate non-knee substitute graft option for ACL repair patients, since it offers a similar outcome to hamstring tendon graft ACL surgery.