Background: Sepsis is a worldwide health problem; it is considered as a form of organ dysfunction. The aim of this study was to identify the role of presepsin in diagnosis of sepsis in ICU patients and its relation to the delta lactate level. Methods: 46 adult participants were included in the study that was conducted at the clinical pathology department and the Intensive Care Unit (ICU) of zagazig university hospitals. The septic group included patients more than 18 years old with positive sepsis criteria that included; temperature > 38°c or < 36°c, leucocytes >12,000/mm3 or < 4000/mm3, heart rate>90bpm or respiratory rate>20 breaths/min. The non-septic group included healthy control individuals above 18 years old. Demographic data were collected from patients. All patients in this study were subjected to presepsin, CRP and WBCs count measurement. Delta lactate was measured using two lactate measurements. Results: We included 31 patients in the septic group; 64.5% (20 participants) of them were males and 15 participants in the non-septic group; 53.3% (8 participants) of them were males. Comparing the two groups regarding sepsis biomarkers; there was a highly significant difference regarding WBCs and CRP with a p-value of