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268351

Radiation-induced effects on genes regulating DNA Repair and Cell Cycle in breast cancer patients.

Article

Last updated: 01 Jan 2025

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Subjects

-

Tags

Medical Oncology

Abstract

Background: Ionizing radiation is a major DNA damaging agent. One of the most common sources of exposure is through medical diagnostics or treatments such as cancer radiotherapy. Aim: This study aims to assess the radiation-induced changes in the expression of DNA repair and cell cycle regulation genes (POLH, PCNA, DDB2, and XPC) in the blood of breast cancer patients and to evaluate their potential as predictive biomarkers for treatment responses. Methods: In this study, the levels of circulating PCNA, POLH, XPC, and DDB2 were evaluated in 51 females: 31 cancer patients and 20 healthy volunteers as a control group. The genes were extracted from whole blood samples and cDNA was synthesized; RT-qPCR was used to assay the expression pattern of these genes. Results: Circulating DDB2 showed a significant difference in the relative expression among the three studied groups. Also, there was a significant difference in the relative expression of circulating DDB2 in the pre-radiotherapy and post-radiotherapy groups relative to the control group. Also, relative to the control group, the circulating levels of the four genes were higher in the pre-radiotherapy group to about double that in the post-radiotherapy group. The four genes combination was capable to discriminate the pre-radiotherapy group from the post-radiotherapy group significantly. Conclusion: The relative expression of these four genes as radiation-responsive genes that are involved in cell cycle regulation and DNA repair could be changed due to radiotherapy. Also, their circulating levels in breast cancer patients could be promising predictive biomarkers for radiotherapy responses.

DOI

10.21608/zumj.2022.165257.2649

Keywords

breast cancer, radiation-responsive genes, Radiotherapy

Authors

First Name

Sameh

Last Name

Nakhla

MiddleName

Fawzy

Affiliation

Radiation Sciences department, Medical Research Institute (MRI), Alexandria University, Alexandria, Egypt.

Email

sameh.nakhlh@alexu.edu.eg

City

-

Orcid

0000-0003-3886-3795

First Name

Rana

Last Name

Khafaga

MiddleName

-

Affiliation

Department of Biochemistry, Medical Research Institute, Alexandria University.

Email

ranahassanmahmoud@alexu.edu.eg

City

-

Orcid

-

First Name

Marwa

Last Name

Gaber

MiddleName

-

Affiliation

Department of Eperimental and Clinical internal Medicine, Medical Research Institute, Alexandria University.

Email

marwa.mahmoud@alexu.edu.eg

City

-

Orcid

-

First Name

Tarek

Last Name

Aref

MiddleName

-

Affiliation

Department of Radiology, Medical Research Institute, Alexandria University

Email

tareftarek@yahoo.com

City

-

Orcid

-

First Name

Medhat

Last Name

Anwar

MiddleName

-

Affiliation

Department of Experimental and Clinical Surgery, Medical Research Institute, Alexandria University

Email

medhat.anwar@alexu.edu.eg

City

-

Orcid

-

First Name

Shaza

Last Name

Saadi

MiddleName

-

Affiliation

Department of Radiology and Medical Imaging, Faculty of Medical Applied Sciences, Al-Azhar University- Palestine

Email

shazawadi0@gmail.com

City

-

Orcid

-

First Name

Esraa

Last Name

Mohamed

MiddleName

-

Affiliation

Department of Radiology, faculty of Applied Medical Science, October 6 University.

Email

esoashraf34@gmail.com

City

-

Orcid

-

First Name

Esraa

Last Name

Gad

MiddleName

-

Affiliation

Department of Radiology, faculty of Applied Medical Science, October 6 University.

Email

esraa.gad512@gmail.com

City

-

Orcid

-

First Name

Amr

Last Name

Hussien

MiddleName

-

Affiliation

Department of Cancer management and research, Medical Research Institute, Alexandria University

Email

amr.hussien@gmail.com

City

-

Orcid

-

First Name

Abdelsalam

Last Name

Ismail

MiddleName

-

Affiliation

Department of clinical oncology, Faculty of Medicine, Alexandria University

Email

abdelsalam.ismail@alexmed.edu.eg

City

-

Orcid

-

First Name

Sawsan

Last Name

Moussa

MiddleName

-

Affiliation

Department of Radiation Science, Medical Research Institute, Alexandria University

Email

sawsan.mousa@alexu.edu.eg

City

-

Orcid

-

Volume

30

Article Issue

1.4

Related Issue

48036

Issue Date

2024-06-01

Receive Date

2022-09-27

Publish Date

2024-06-01

Page Start

275

Page End

285

Print ISSN

1110-1431

Online ISSN

2357-0717

Article File

ZUMJ_Volume 30_Issue 1.4_Pages 275-285.pdf

PDF . 756.6kB

Link

https://zumj.journals.ekb.eg/article_268351.html

Detail API

https://zumj.journals.ekb.eg/service?article_code=268351

Order

25

Type

Original Article

Type Code

273

Publication Type

Journal

Publication Title

Zagazig University Medical Journal

Publication Link

https://zumj.journals.ekb.eg/

MainTitle

Radiation-induced effects on genes regulating DNA Repair and Cell Cycle in breast cancer patients.

Details

Type

Article

Created At

30 Dec 2024