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Tissue ELISA and Serum Amperometric Magnetoimmunosensor of ECD-HER2 In Stratifying Subtyping of Egyptian Woman Breast Cancers

Article

Last updated: 01 Jan 2025

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Tags

Medical Oncology

Abstract

Background: Detection of HER2 extracellular domain (ECD) amperometric magnetoimmunosensor (AM) investigated on raw cell lysates, intact breast cancer cells, and commercial and healthy sera except on patients' samples.
Aim: The current study aimed to validate tissue and serum AM of ECD-HER2 in subtyping Egyptian woman breast cancers and to compare it to the current techniques.
Subjects and Methods: We collected blood and tissues from 72 women (20 controls, five recurrent breast tumors, and 47 BCs). Tissue (tHER2) and serum HER2 (sHER2); are detected by ELISA and the AM. Results correlated with the clinicopathological parameters, and serum CA15-3 (sCA15-3).
Results: tHER2 (ELISA) and sCA15-3 significantly differed among the studied groups. tHER2 (ELISA) showed a significant decrease in higher BMI in the HER22+ group, while tHER2 and sHER2 (AM) significantly correlated in the HER23+ group. tHER2 (ELISA) significantly differed among BCs molecular subtypes. Both tHER2 and sHER2 (ELISA) significantly correlated in the HER2 molecular subgroup. sHER2 (AM) showed highly significant sensitivity and specificity in differentiating TN from luminal A at > 9.26 ng/ml and HER2 at 9.6 ng/ml. However, at > 1.67 ng/ml, tHER2 (ELISA) stratified significantly between BCs molecular and HER2 subgroups.
Conclusion: Tissue ELISA and serum AM provided accurate quantitative measurements for HER2 in various BC subtypes—at cut-off values lower than those approved by FDA—and would complement the current IHC and ISH techniques.

DOI

10.21608/zumj.2022.161766.2643

Keywords

Breast carcinoma, CA 15-3, HER2, Immunoassay, immunosensors

Authors

First Name

Sameh

Last Name

Nakhla

MiddleName

Fawzy

Affiliation

Radiation Sciences department, Medical Research Institute (MRI), Alexandria University, Alexandria, Egypt.

Email

sameh.nakhlh@alexu.edu.eg

City

-

Orcid

0000-0003-3886-3795

First Name

Zynab

Last Name

Elwafe

MiddleName

-

Affiliation

2. Radiology Department, Faculty of Medical Technology, Derna, Libya

Email

z77zekry@yahoo.ca

City

-

Orcid

-

First Name

Ahmed Saad

Last Name

Saad

MiddleName

-

Affiliation

Experimental and Clinical Surgery Department, MRI, Alexandria University, Alexandria, Egypt.

Email

ahmed41061@hotmail.com

City

-

Orcid

-

First Name

Tarek

Last Name

Aref

MiddleName

-

Affiliation

Radiodiagnosis Department, MRI, Alexandria University, Alexandria, Egypt.

Email

areftarek@yahoo.com

City

-

Orcid

-

First Name

Noha

Last Name

Ragab

MiddleName

-

Affiliation

Department of Pathology, MRI, Alexandria University, Alexandria, Egypt

Email

drnoharagab@live.com

City

-

Orcid

-

First Name

inas

Last Name

elbadry

MiddleName

-

Affiliation

Department of Cancer Management and Research, MRI, Alexandria University, Alexandria, Egypt

Email

dr.inaselbadry@gmail.com

City

-

Orcid

-

First Name

Unai Eletxigerra

Last Name

Eletxigerra

MiddleName

-

Affiliation

Micro-NanoFabrication Unit, IK4-Tekniker, Eibar, Spain

Email

unai.eletxigerra@tekniker.es

City

-

Orcid

-

First Name

Susana

Last Name

Campuzano

MiddleName

-

Affiliation

Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, Madrid, Spain.

Email

susanacr@quim.ucm.es

City

-

Orcid

-

First Name

Jesus

Last Name

Martínez-Perdiguero

MiddleName

-

Affiliation

Universidad del País Vasco/Euskal Herriko Unibertsitatea, Bizkaia, Spain.

Email

jesus.martinez@ehu.eus

City

-

Orcid

-

First Name

Santos Merino

Last Name

Merino

MiddleName

-

Affiliation

Micro-NanoFabrication Unit, IK4-Tekniker, Eibar, Spain

Email

santos.merino@tekniker.es

City

-

Orcid

-

First Name

José

Last Name

M. Pingarrón

MiddleName

-

Affiliation

Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, Madrid, Spain.

Email

pingarro@quim.ucm.es

City

-

Orcid

-

First Name

Salah

Last Name

Abdelmoneim

MiddleName

-

Affiliation

Department of Clinical Oncology and Nuclear Medicine, faculty of medicine, AlexandriaUniversity, Alexandria, Egypt.

Email

salah.elmoneim@alexmed.edu.eg

City

-

Orcid

-

First Name

Eman

Last Name

El-Abd

MiddleName

-

Affiliation

Radiation Sciences department, Medical Research Institute (MRI), Alexandria University, Alexandria, Egypt.

Email

iman.elabd@alexu.edu.eg

City

-

Orcid

-

Volume

30

Article Issue

1.4

Related Issue

48036

Issue Date

2024-06-01

Receive Date

2022-09-11

Publish Date

2024-06-01

Page Start

141

Page End

149

Print ISSN

1110-1431

Online ISSN

2357-0717

Article File

ZUMJ_Volume 30_Issue 1.4_Pages 141-149.pdf

PDF . 901.9kB

Link

https://zumj.journals.ekb.eg/article_264264.html

Detail API

https://zumj.journals.ekb.eg/service?article_code=264264

Order

13

Type

Original Article

Type Code

273

Publication Type

Journal

Publication Title

Zagazig University Medical Journal

Publication Link

https://zumj.journals.ekb.eg/

MainTitle

Tissue ELISA and Serum Amperometric Magnetoimmunosensor of ECD-HER2 In Stratifying Subtyping of Egyptian Woman Breast Cancers

Details

Type

Article

Created At

30 Dec 2024