Background: Infectious diseases caused by viruses that are commonly found in the liver include hepatitis C virus (HCV), SEN virus (SENV), and the Teno virus (TTV). This work aimed to find out the frequency of SENV & TTV infections and to correlate their viral load with their clinical condition among hemodialysis patients in Zagazig University Hospitals.

Subjects and methods: In a case-control study we included 44 patients divided into 2 groups; 22 hemodialysis patients attending to hemodialysis unit and 22 apparently healthy individuals served as control group. Detection of anti HCV antibodies & anti HBsAg was done by ELISA. Detection of SENV DNA and TTV as done by nested PCR analysis.

Results: Statistically significant differences were found between the studied groups regarding prevalence of SEN D, SEN H, coinfection SEN D and H, TTV and combined SEN/TTV genotypes (p=0.048, 0.021, 0.048, 0.026, and 0.021 respectively). SEN D, SEN H, TTV prevailed in 22.7%, 45.5% and 50% of patients on HD. Combined SEN V D and H prevailed in 22.7% while 45.5% of HD patients had coinfection with SENV and TTV. Also, statistically significant differences were revealed between the studied groups regarding prevalence of combined SEN H/D and TTV genotypes among hemodialysis patients and comorbid hypertension, diabetes ALT, AST, and hemodialysis duration (p=0.004, <0.001, 0.002,0.002 and 0.008 respectively).

Conclusion: We found a significantly higher prevalence of SEN virus D, SEN virus H, TTV, and concurrent infections involving combinations of these viruses among hemodialysis patients compared to healthy controls. T