Background Unconjugated hyperbilirubinemia is the most common cause of hospital admissions and readmissions among the newborns. Aim of the work: Levels of hydrogen peroxide in cord blood can be used to confirm the predictive warning sign of neonatal hyperbilirubinemia and identify which babies require follow-up leaving the hospital. Patients and methods a cross-sectional study involved 30 healthy neonates. They were classified into two groups according to bilirubin level at the 5th day: less severe hyperbilirubinemia with a bilirubin level ≤ 17 mg/dl and severe hyperbilirubinemia with a bilirubin level > 17 mg/dl. Measurement of hydrogen peroxide in cord blood, at birth, and on the third and fifth days of life by colorimetric method. Results in our study show that at the cutoff point of cord blood hydrogen peroxide ≥2.685 uMol/L, sensitivity was 90.0%, specificity was 83.3%, predicted values (both positive and negative) were 78.3% and 92.6%, positive and negative likelihood ratios were 5.4 and 0.12, and accuracy was 86.0%. Conclusion: The levels of cord hydrogen peroxide and further sampling were considerably greater in neonates with severe hyperbilirubinemia. It is therefore possible to identify a subset of newborns who may be at risk of jaundice and require phototherapy or exchange at delivery by measuring the amounts of hydrogen peroxide in the umbilical cord blood.