Background: Hysterectomy is the most common non-obstetrical surgical procedures among women. This study aimed to assess the effect of hysterectomy with ovarian conservation on ovarian function. Methods: This cross-sectional observational study was conducted in Obstetrics and Gynecology Department of Zagazig University Hospital on 80 cases scheduled to undergo hysterectomy with ovarian preservation due to benign condition during the period from August 2015 to August 2018. Participants were subjected to assessment of ovarian function by ultrasonographic assessment of ovarian volume, morphology and doppler indicies and hormonal levels pre-operative and postoperative. The research was accepted by the Institutional Review Board of the Faculty of Medicine-Zagazig University. The study was done according to the Code of Ethics of the World Medical Association (Declaration Helsinki) for Studies involving humans. Results: There is statistically non-significant decrease in FSH level preoperatively to one month postoperatively that significantly increased again three months postoperatively.. There is non-significant diffrence between right ovarian volume preoperatively and one month postoperatively. There is significant difference between right ovarian volume preoperatively and three months postoperatively. Conclusions: there is no evidencve of ovarian dysfunction affecting conserved ovaries 3 months after hysterectomy in premenopausal women as evident by hormonal and U/S criteria.