Background: The operation of resecting an ovarian cyst (ovarian cystectomy) can be performed either by laparotomy or laparoscopy. Recent studies have reported significant decrease in ovarian reserve, estimated by measurement of serum anti-mullerian hormone (AMH) levels drops significantly after ovarian cystectomy. The aim of this work was the comparison of anti-mullerian hormone level in benign ovarian cyst Before and after laparoscopic ovarian cystectomy. Methods: This was prospective observational study in the period from August 2020 to December 2020. Included 34 women at Obstetrics and Gynecology Department, Zagazig University Hospital undergoing Laparoscopic Ovarian Cystectomy for benign ovarian cyst to evaluate the ovarian reserve by using AMH level determination, and at one and 3 months after laparoscopic cystectomy. Results: There was significant higher reduction in AMH among younger than older age groups (49.7% versus 41.5%), bigger than smaller cyst size (51.5% versus 39.2%), higher reduction among patients with higher pre-operative Anti-Mullerian Hormone (≥ 5 ng/ml) than