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284072

Histopathological Evaluation of Iron and Iron chelator Deferoxamine in Experimentally Infected Mice with Toxoplasma gondii

Article

Last updated: 01 Jan 2025

Subjects

-

Tags

Medical Parasitology

Abstract

Toxoplasmosis is a worldwide parasitic disease infecting about one third of human population. With the prevalence of drug-resistant strains on the rise, new therapies for toxoplasmosis are urgently required. Little studies have been ‎managed to evaluate the effect of iron and iron chelators in experimental toxoplasmosis. Therefore, we investigated if the iron chelator, Deferoxamine can affect Toxoplasmosis outcome in experimentally infected mice. Eighty Swiss albino mice were classified into four groups (20 mice each) as follows: (GI): Healthy control group; (GII): infected non-treated control; (GIII): infected iron supplemented group; (GIV): infected deferoxamine treated group. Sacrifice of mice was performed on day 8 post infection for measuring the serum iron level and for histopathological analysis of intestinal tissue samples. Our data indicated that infected-iron supplemented group (GIII) was associated with more increase in the mean serum iron level (150μg/dl). However, the mean serum iron level in deferoxamine treated group (GIV) decreased (77.2μg/dl) with highly statistically significant difference. Those findings were confirmed by histopathological assessment; the infected iron supplemented group (GIII) exhibited marked histopathological changes in the intestinal tissues of experimentally infected mice that were greatly improved in GIV after chelation of iron using deferoxamine treatment. Finally, the present work showed that the acquisition of host iron by T.gondii is a critical step in the progression of infection and is determinant in its outcome. Therefore, deferoxamine could be a promising drug to control acute T.gondii infection due to the limitation of iron availability, and further research is necessary to elucidate these points.

DOI

10.21608/zumj.2023.190273.2738

Keywords

T.gondii, iron, Deferoxamine, serum iron, histopathology

Authors

First Name

Enas

Last Name

Elbahaie

MiddleName

-

Affiliation

Medical Parasitology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

Email

enas.elbahaie@gmail.com

City

Zagazig

Orcid

0000-0002-3439-9369

First Name

Faten

Last Name

Mohammed

MiddleName

-

Affiliation

Medical Parasitology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

Email

fatenmr@yahoo.com

City

Zagazig

Orcid

-

First Name

Alia

Last Name

Ibrahim

MiddleName

Ibrahim Mohamed

Affiliation

Medical Parasitology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

Email

ibrahembasma904@gmail.com

City

Zagazig

Orcid

-

First Name

Eman

Last Name

Mostafa

MiddleName

-

Affiliation

Medical Parasitology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

Email

emanmostafa81@gmail.com

City

Zagazig

Orcid

-

Volume

30

Article Issue

1

Related Issue

44990

Issue Date

2024-01-01

Receive Date

2023-02-01

Publish Date

2024-01-01

Page Start

241

Page End

247

Print ISSN

1110-1431

Online ISSN

2357-0717

Link

https://zumj.journals.ekb.eg/article_284072.html

Detail API

https://zumj.journals.ekb.eg/service?article_code=284072

Order

25

Type

Original Article

Type Code

273

Publication Type

Journal

Publication Title

Zagazig University Medical Journal

Publication Link

https://zumj.journals.ekb.eg/

MainTitle

Histopathological Evaluation of Iron and Iron chelator Deferoxamine in Experimentally Infected Mice with Toxoplasma gondii

Details

Type

Article

Created At

30 Dec 2024