The Value of Concomitant Measurement of miR 323b-5p and miR 486-5p with Ankle Brachial Index in Diagnosis of Peripheral Arterial Disease

Background: Peripheral artery disease (PAD) is an advanced atherosclerotic disease causing augmented risks of hospitalization and death. So, several studies tried to detect novel biomarkers for PAD diagnosis.

Aim of the study: is to explore the role of plasma miRNA 323b-5p and miRNA 486-5p levels to diagnose PAD.

Methods: The research was performed in Medical Biochemistry & Cardiology Departments – Zagazig Faculty of Medicine. It included 100 volunteers categorized into 2 groups; Group (1): included 50 healthy volunteers served as control group with ankle brachial index (ABI) > 0.9. Group (2): included 50 PAD patients with ABI < 0.9. Plasma 323b-5p and miR 486-5p expression level were measured via real time PCR. Color Doppler was performed to calculate ABI, which was correlated with miRNA levels.

Results: There was highly significant difference between groups as regards miR 323b-5p. Furthermore, miR 486-5p expression level showed significant variance between groups. Regarding miRNA 323b-5p expression level, cutoff value was 2.55, sensitivity was 89.3% and specificity was 72.7%. Concerning miR 486-5p expression level, cutoff value was 1.92, sensitivity was 82.1% and specificity was 81.8%. As regards combined miR 323b-5p and miR 486-5p ROC curve, it was 96.4% sensitivity and 95.5% specificity. Each miRNA showed negative correlation with ABI.

Keywords: ABI, PAD, real time-PCR, miRNA323b-5p, miRNA 486-5p.