Background &Aim : Ankylosing spondylitis (AS) is a chronic inflammatory condition that affects the sacroiliac, and peripheral joints. Systolic dysfunction in AS by conventional assessment may not detect early impairment, so we aimed to assess two-dimensional (2D)-speckle tracking echocardiography (STE) on the left ventricle (LV) and right ventricle (RV) and correlate these findings with AS disease activity score (ASDAS-CRP). Methodos: A case-control study involving 135 participants; they were classified into three groups, inactive group (I) 57 cases, active group (II) 33 cases, and 45 control group (III) . The (ASDAS-CRP) score was calculated for all patients. Laboratory parameters ;c–reactive protein (CRP), and lipid profile , conventional echocardiographic assessment of ventricular systolic function and (2D) (STE) on both (LV) and (RV) were done. Results: (ASDAS-CRP) score was high in the the active group (II) with a p-value <0.001. Right ventricular free wall strain (RVFWS) was more impaired in the same group p-value <0.001. Pulmonary artery systolic pressure (PASP) was also high in the same group p-value <0.001. AS population was divided into 2 subgroups according to drug type biological 70 cases versus non-biological 20 cases. RVFWS was more impaired in the biological drug group (-18.35%±3.08 versus -20.76±2.21 in the non-biological group, p-value 0.002. The predictor of impaired RVFWS in AS was PASP (p= 0.001), Exp (B) 1.143, 95% C.I: (6.895 to 13.714). RVFWS had a significant negative correlation with (ASDAS-CRP), disease duration, and PASP Conclusion: Impaired RVFWS was detected in patients with AS and was associated high disease activity score.