Abstract
Background: Recently there are contradictory reports on the effects of sex hormones on pathogenesis of IBS, so many researches were held to clarify the relation between the level of sex hormones and IBS .
Aim of the study: to examine the effect of female sex hormones on the inflammatory signs present in serum and changes in GIT associated with chronic IBS stress in rats.
Material, Methods:60 healthy female of local strain group were divided equally into 6 groups: Group1:OVX Sham group , Group2:OVX sham group With IBS, Group3: OVX IBS group, Group4 OVX IBS Estrogen treated group , Group5: OVX IBS Progestin treated group , Group6: OVX IBS estrogen &Progestin treated group . after OVX hormones were given for continues 14 days and rats were submitted to chronic stress exercise for 10 days ,and after the last dose of replacement hormones serum histamine, serotonin and IL8 levels were determined , distal colon was processed for histopathology.
results :there was a significant increase in serum IL8, histamine with marked drop in serotonin level in both IBS and IBS OVX groups compared to control and hormonal treated groups. additionally, there was a marked disturbance in colonic mucosal structure with elevated claudin 1 protein level in both IBS and OVX IBS groups compared to other groups.
Conclusion: female replacement hormones were found to attenuate inflammatory markers and mucosal disturbance associated with IBS.