Background: Glucocorticoids are used in the treatment of several diseases, but they have many side effects. many actions for vitamin D have been discovered due to its strong antioxidant properties. Objective: The aim of the present study was to detect the protective effect of vitamin D against glucocorticoids induced metabolic syndrome and gastric lesion. Materials and methods: 24 adult male wistar albino rats were included. Rats were randomly allocated into 4 groups (6 rats/each group), group1 (control), group 2 (vitamin D treated ), group 3 (Dexamethasone treated ) and group 4 (dexamethasone with vitamin D treated ). Drugs were given intraperitoneally daily for 14 days. Blood pressure (BP) was measured before and after the experiment. Serum glucose, insulin, Lipid profile were measured. Also, angiotensin receptor 1 (AT1) gene expression in aortic tissue was detected. Histopathological Analysis for stomach was performed. Results: Dexamethasone resulted in a significant increase of BP, glucose, insulin and dyslipidemia with an increase of AT1 gene expression and a significant hypocalcemia was detected besides gastric lesions. Giving vitamin D with dexamethasone reversed these results. Conclusion: Vitamin D prevented dexamethasone induced metabolic syndrome and gastric lesion due to its antioxidant properties besides inhibition of AT1 gene expression.