Background: The diabetes mellitus (DM) prevalence was 525 million in 2019 and type 2 (T2DM) around 90% of all cases of diabetes. Diabetic neuropathy (DN) is a micro-vascular complication of DM and considered a major cause of mortality and morbidity in diabetic population. The aim of this study to evaluate the relation between a possible vitamin D level and diabetic neuropathy development. Patient and methods: A study included 80 patients divided into 4 groups; (A): diabetic patients with painful diabetic neuropathy; (B): diabetic patients with painless diabetic neuropathy; (C): diabetic patients with painless neuropathy and neuropathic ulcer and (D) healthy controls. Serum vitamin D levels was measured in all patients and neurological scoring for neuropathy was assessed. Results: Patients In group A had neuropathy score from 9 to 15. Patients in group B had Neuropathy score from 7 to14. In group C, patients had neuropathy score from 6 to 9. 60% of group A had deficient vitamin d level. 65% of group B of had deficient vitamin d level. 5% only of the studied healthy control had deficient vitamin D. There was highly statistically significant difference between the four groups regarding vitamin D level and vitamin D status assuming that vitamin D deficiency was below 25 ng/dl. Conclusion: Vitamin D deficiency is considered as major risk factor for development of diabetic peripheral neuropathy (DNP). Therefore, we recommended to estimate vitamin D level in patients with DPN.