Abstract Background: Glioblastoma Multiforme (GBM) is one of the most fatal of all brain tumors. TP53 mutation is the hall-mark genetic mutation of GBM, because these tumors have a high incidence of mutation in this gene (>65%), suggesting that p53 pathway has an important role in the development of the tumors. Aim of Study: This study aimed to assess the prevalence of TP53 gene mutation in Glioblastoma multiforme patients and its correlation to the clinical pictures of this tumors in Egypt. Patients and Methods: This retrospective cross-sectional study included 59 patients pathologically confirmed of having GBM after surgical excision, underwent further immunological analysis of presence of TP53 gene mutation. Results: Patients with TP53 presented with DCL (p-value =0.703), fits (p-value=0.677), weakness (p-value=0.212) were statistically insignificant. Conclusion: Patients diagnosed with TP53 gene mutation didn't present with significantly different clinical picture from other patients.