This study was carried out at the Plant Tissue Culture Laboratory and Greenhouse of the Department of Horticulture, Faculty of Agriculture, Suez Canal University, Ismailia Governorate, Egypt, during the period of 2017 – 2022. The main aims of the current study were to establish an efficient protocol for in vitro propagation of vegetable rootstocks and to compare the performance of rootstocks produced in vitro and those produced directly from seeds (in vivo), in terms of growth, yield and quality parameters. Two commercial rootstocks, namely Super Shintoza and Forza for Cucurbits and tomato grafting, respectively, were used. Also, the cucumber Hayel variety and tomato Assila F1 (T-186) were used as scions. The rootstock seeds of both genotypes were used as explants. The results showed that the micropropagation technique can be used efficiently to produce in vitro rootstocks, especially for tomato rootstocks, in order to decrease the high cost of the interspecific rootstocks for commercial production of grafted vegetables.