Utilizing grape rootstocks is considered an efficient method to enhance the resistance against biotic and abiotic stresses. Therefore, a pot experiment was conducted during Two consecutive seasons 2020 and 2021.to evaluate the responses of 1-year-old rooted cuttings of commercially available grape rootstocks (SO4, Freedom, Ramsey, and Richter) planted in sandy soil, amended with compost; then irrigated with graduated levels of salt mixture solution (control, 1500, 3000, 4500, and 6000 ppm). The results obtained from biochemical and physiological measurements revealed that Ramsey rootstock outperformed the other rootstocks, significantly exhibiting the highest concentrations of nitrogen, potassium, magnesium, and proline in roots, while demonstrating the lowest sodium concentration in petioles. The results offer valuable insights on grape cultivation under salinity conditions with irrigation water concentration ranging from 1,500 to 3,000 ppm.