A protocol was developed for micropropagation of Quisqualis indica (Combretaceae). Media for culture establishment, multiplication, rooting and hardening were studied. For culture establishment and multiplication, MS medium supplemented with BAP 1.5 mg/l plus GA3 1 mg/l produced the highest number of shoots/explant (3.43) among the various tested concentrations of BAP and GA3. For rooting, ½ MS medium without any growth regulators was effective and formed more number (6.00) and longer roots (13.00 cm). Plantlets were successfully acclimatized in a mixture of perlite, peat-moss and vermiculite 1:1:1 v/v/v under ex-vitro conditions with a survival rate of 73.3 %.