Carbon nanotubes application attracts researcher's attention due to benefits occurred. The safest way to benefit from nanotechnology's advantages and stay away from the majority of its drawbacks is to use nanomaterials in controlled in-vitro experiments. This study describes the effect of carbon nanotubes (CNTs) on regeneration of pineapple cultures, its active ingredients and antioxidant activities. Carbon nanotubes were used at 0.0, 0.2, 0.02 and 0.002 g/l to study their effect on pineapple multiplication and rooting stages. Results showed that CNTs treated cultures showed higher shoot multiplication rate as its concentration at 0.2 g/l gave the highest shoot number followed by 0.02 and 0.002 g/l compared with the control treatment (0.0 g/l). It is worth mentioning that all plantlet parameters, in rooting stage, showed significant higher results with CNTs compared with the control. As for the chemical composition analysis, the positive effect of CNTs was reversed by increasing the application rate. It is assumed that the low level of carbon nanotubes used has a role as an antioxidant due to their radical capacity. These results clearly encourage the application of carbon nanotubes with low concentration as a potent natural candidate for antioxidation.