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382400

Elicitation of gentiopicroside in Gentiana kurroo Royle shoot cultures with endophytic fungus

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Last updated: 01 Jan 2025

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Abstract

Gentiopicroside (GPD) is a major secoiridoid glucoside in the herbs of the Gentianaceae family, predominantly in the genus Gentiana. Gentiana kurroo Royle, a native of the Indian Himalayas is rich in GPD in the roots and shoots. It is used extensively for treating various diseases in Ayurvedic medicine. This herb grows above 1600–3500 Masl and is endemic to the Himalayan region and extensively harvested from wild sources for its immense medicinal properties. In this study, in vitro shoot cultures of G. kurroo were stimulated with Trichoderma viride, Aspergillus niger, Fusarium oxysporum, and an endophytic fungal cell wall extract for enhanced production of GPD. The results showed that treatment with the endophytic fungal extract produced a much higher concentration of GPD (30.4 mg/g DW) after 48 hrs of treatment compared with other treatments. The endophytic fungus was identified as Parengyodontium album. This study provides a simple and efficient method for in vitro elicitation of GPD.

DOI

10.21608/ejbo.2024.240513.2582

Keywords

fungal elicitors, Endophyte, Gentiana kurroo, gentiopicroside, in vitro shoots

Authors

First Name

Mariadoss

Last Name

Alphonse

MiddleName

-

Affiliation

Vellore Institute of Technology

Email

dosssaga@gmail.com

City

Vellore

Orcid

0000-0002-5154-365X

First Name

Kalaivani

Last Name

Thiagarajan

MiddleName

-

Affiliation

1Department of Biotechnology, School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore – 632014, TamilNadu, India

Email

tkvani72@gmail.com

City

Vellore

Orcid

0000-0001-8304-9376

First Name

Rahda

Last Name

Navapara

MiddleName

-

Affiliation

Vellore Institute of Technology

Email

radha.patel8822@gmail.com

City

-

Orcid

-

First Name

Richa

Last Name

Doshi

MiddleName

-

Affiliation

Vellore Institute of Technology

Email

dricha.idar@gmail.com

City

-

Orcid

-

First Name

Nidhi

Last Name

Sabade

MiddleName

-

Affiliation

Vellore Institute of Technology

Email

nidhisabade143@gmail.com

City

-

Orcid

-

First Name

Gholamreza

Last Name

Abdi

MiddleName

-

Affiliation

Department of Biotechnology, Persian Gulf Research Institute, Persian Gulf University, Bushehr, 75169, Iran

Email

abdi@pgu.ac.ir

City

Bushehr

Orcid

0000-0002-1983-4369

First Name

Sudhir

Last Name

Singh

MiddleName

-

Affiliation

Plant Biotechnology and Secondary Metabolites Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai, 400 085, India Homi Bhabha National Institute, Anushaktinagar, Mumbai, 400094, India

Email

sudhirs@barc.gov.in

City

Mumbai

Orcid

0000-0001-9210-4605

First Name

Devanand

Last Name

Fulzele

MiddleName

P

Affiliation

Plant Biotechnology and Secondary Metabolites Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai, 400 085, India

Email

dfulzele@gmail.com

City

Mumbai

Orcid

-

First Name

Michael

Last Name

Pillay

MiddleName

-

Affiliation

Department of Life and Consumer Sciences, College of Agriculture and Environmental Sciences, University of South Africa, Florida Campus, Johannesburg, South Africa

Email

pillam@unisa.ac.za

City

Johannesburg

Orcid

-

First Name

Siva

Last Name

Ramamoorthy

MiddleName

-

Affiliation

School of Bio Sciences and Technology, VIT University, Vellore 632014, Tamil Nadu, India

Email

siva.ramamoorthy@gmail.com

City

Vellore

Orcid

0000-0001-7509-8602

First Name

Rajasekaran

Last Name

Chandrasekaran

MiddleName

-

Affiliation

Vellore Institute of Technology

Email

drcrs70@gmail.com

City

Vellore

Orcid

0000-0002-1553-0907

Volume

64

Article Issue

4

Related Issue

50599

Issue Date

2024-12-01

Receive Date

2023-11-28

Publish Date

2024-12-01

Page Start

120

Page End

126

Print ISSN

0375-9237

Online ISSN

2357-0350

Article File

EJBO_Volume 64_Issue 4_Pages 120-126.pdf

PDF . 1.7MB

Link

https://ejbo.journals.ekb.eg/article_382400.html

Detail API

https://ejbo.journals.ekb.eg/service?article_code=382400

Order

10

Type

Special Issue (Original Article)

Type Code

2,866

Publication Type

Journal

Publication Title

Egyptian Journal of Botany

Publication Link

https://ejbo.journals.ekb.eg/

MainTitle

Elicitation of gentiopicroside in Gentiana kurroo Royle shoot cultures with endophytic fungus

Details

Type

Article

Created At

30 Dec 2024