This study focused on Acacia nilotica because of the plant's long history of ecological and folk medical applications. The Phytochemical compounds of Acacia nilotica were examined with purpose to find the antibacterial actions of this plant. Methanol was used to extract on dried Acacia nilotica fruit. The phytochemical screening conducted out on the Acacia nilotica fruit extract indicated high quantity of phenolic, Flavonoids and Tannin in methanol extract. Pods was analyzed by HPLC, there were 16 phenolic compounds rich in gallic acid as a major phenolic compound (24048.50 µg/g) and catechin as a major flavonoid (66162.88 µg/g). Seven commonly studied bacteria strains (Escherichia coli, Bacillus subtilis, Bacillus cereus, Salmonella typhimurium, Staphylococcus aureus, Enterobacter cloacae, and Klebsiella pneumoniae) were used to test the extract's antimicrobial properties. Antimicrobial testing revealed that the methanolic extract inhibited the development of every type of bacterium tested. The results may encourage baseline information for prospective use of these crude extracts in medication development programs in the pharmaceutical sectors as an example