The present experiment was undertaken to studying the behavior of titanium in the soil and the effect of application methods (foliar spraying or soil injection) and TiO2 concentrations (0, 5, 7.5, 10ppm as a nanoparticles or 25, 50 and 75ppm as a bulk particles) as well as their interaction treatments on Salvia fruticosa (Three-lobed sage) plant. In most cases, the tallest plants, the maximum yield of dry herb / feddan, the maximum yield of volatile oil / feddan and the maximum values of chlorophyll and NPK of Salvia fruticosa were achieved from the interaction treatment between foliar spraying and 10ppm TiO2 nanoparticles. While, the main components of three-lobed sage essential oil were 1,8 Cineole , Camphor and α–pinene and camphene respectively. Also, from sequential extraction method, the highest fraction of Ti was found in residual forms at the each of all treatments. This confirmed that using TiO2 (bulk or nanoparticles) whether soil or foliar application is harmless and improved chlorophyll production and consequently improved photosynthesis under this study.