Purpose: This current research is motivated by the activation of copper metals and their harmful effects on the economic crop barley 132 (Hordeum vulgaris). Methods: Grains were grown for 21 days in pot experiments under different concentrations of copper (control, 5, 10, 15, and 25 mM). To test the impact of copper, we estimated its morphological and physiological traits. Results: Growth parameters were enhanced with all copper concentrations except 25 mM, which showed a decreasing trend. The best concentration for this activation was 15 mM compared to the control. Copper treatments showed greenish enlarged leaves and elongated roots and shoots. As associated with plants irrigated with H2O, soluble sugar, soluble protein, and total protein in shoots increased with copper supplementation. Additionally, cellulose and phenols were improved markedly with increased copper concentrations. Besides, catalase and peroxidase enzymes were enhanced at 10 and 15 mM concentrations. Catalase and peroxidase profiles showed three varied bands at control, 10, and 25 mM. The protein pattern at a concentration of 10 mM displayed mostly similarity to the control, while at 25 mM concentration, a significant aggregation of protein bands was observed. Conclusion: Copper acts as an activator at low concentrations, while higher concentrations act as inhibitors. Results provided a baseline for further research on the mechanism of copper activation in the barley crop.