Sesamum phyllody is a severe disease that displays various symptoms such as floral virescence, phyllody, proliferation, yellowing, cracking of seed capsules, and the formation of dark exudates on foliage. Infected plants were found to have blue areas in the phloem region using Diane's stain, and phytoplasma structures in the phloem sieve elements were detected using transmission electron microscopy. The amplification of a phytoplasma-specific 1200-bp 16S recombinant DNA (rDNA) fragment confirmed that the plants were infected with a phytoplasma. To regenerate phyllody-free plantlets from meristem tips, Murashige and Skoog medium was utilized and phytoplasma indexing was performed using nested PCR (nPCR) on the mother plant and the in vitro-regenerated plantlets. In an effort to produce phyllody-free plants, explants from phyllody-positive Sesamum plants were cultured in Murashige and Skoog medium with varying concentrations of silver nanoparticles (Ag NPs). As the concentration of Ag NPs increased up to 300 mg L^-1, shoot regeneration was reduced. However, in vitro-regenerated plantlets at a concentration of 250 mg L^-1 were tested negative for phyllody and proved to be phyllody free. The most effective concentration was found to be 250 mg L^-1, resulting in 100% phyllody-free plants and 100% of cultures showing shoot regeneration. Thus, treatment with Ag NPs is a promising technique for producing phyllody-free Sesamum plants.