Numerous herbal plants have been examined for their significant therapeutic potential as antioxidants and anticancer agents, as well as their ability to support a non-specific immune system against infections and other related diseases. In the present study, DPPH scavenging assay and cytotoxicity were used to investigate the correlation between the antioxidant activity of herbal plants and MCF-7 breast cancer cells, with minimal effect on normal mammalian kidney cells. Using the MTT assay, the IC50 values for rosemary, sage, ginger, and thyme to inhibit cell proliferation were 37.05, 93.09, 75.64, and 92.38 µg/ml, respectively. In contrast, the IC50 values for DPPH scavenging for ascorbic acid, sage, rosemary, ginger, and thyme were 9.51, 10.03, 12.01, 3.66, and 4.956 µg/ml, respectively. The experimental animals demonstrated the ability of the ethanolic extracts to ameliorate hematological changes by increasing signs of the total count of white blood cells (WBCs) and lymphocytes compared with the cyclosporine group. This result is attributed to the treatment of rats with Cyclosporine-induced significant decrease in total counts and lymphocytes. Quantification of TNF-α and IFN-γ cytokines as a mediator of the immune system revealed an increase in their expression levels in groups of co-administrated sage and rosemary extracts. The present study aimed to evaluate the immunomodulatory and cytotoxicity activity of thyme, sage, ginger, and rosemary ethanolic by inducing oral administration of cyclosporin as an immunosuppressive agent in vivo and cell cytotoxicity on healthy and cancerous cells in vitro.