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300926

Effect of Abscisic acid on Legume Growth Rate, Seed Development and Protein Profile in <i>Pisum sativum</i> L.

Article

Last updated: 01 Jan 2025

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Abstract

In this work, cultivated pea (Pisum sativum L.cv. Master B) is used as a legume model crop plant to investigate the effect of abscisic acid (ABA) treatment on seed filling during embryogenesis. This was evaluated by using two exogenously applied concentrations (10& 20 µM) during three stages of seed developments (early, intermediate and late). Phenotypic characterization of the treated plants included measurement of seed / legume length and diameter at full maturation stages after treatment. The expression of major proteins of the treated plants was detected using SDS-PAGE electrophoresis technique. Our data showed a positive effect of ABA when applied at the late stage of seed development. ABA significantly increased legume size and elevated the expression of some major proteins such as convicilin (70-75 kDa), vicilin (50, 34 kDa) and legumin (40 kDa). The impact of these results is further discussed. 

DOI

10.21608/ejbo.2023.202685.2295

Keywords

ABA, embryogenesis, Pisum sativum, SDS- PAGE, Seed filling

Authors

First Name

Nada A.

Last Name

El-Taweel

MiddleName

-

Affiliation

Molecular Genetic Unit, Botany Department, Faculty of Science, Ain Shams University, Cairo, Egypt

Email

nada.eltawel@hotmail.com

City

Cairo

Orcid

0000-0002-0081-4229

First Name

Mervat Sh.

Last Name

Sadak

MiddleName

-

Affiliation

Botany Department, Agricultural and Biological Institute, National Research Center, Dokki, P.O. 12622, Giza, Egypt

Email

mervasha@hotmail.com

City

Cairo

Orcid

0000-0002-0081-4229

First Name

Noha S.

Last Name

Khalifa

MiddleName

-

Affiliation

Molecular Genetic Unit, Botany Department, Faculty of Science, Ain Shams University, Cairo, Egypt

Email

nohakhalifa@hotmail.com

City

-

Orcid

0000-0002-0081-4229

Volume

63

Article Issue

3

Related Issue

43286

Issue Date

2023-09-01

Receive Date

2023-03-28

Publish Date

2023-09-01

Page Start

969

Page End

980

Print ISSN

0375-9237

Online ISSN

2357-0350

Link

https://ejbo.journals.ekb.eg/article_300926.html

Detail API

https://ejbo.journals.ekb.eg/service?article_code=300926

Order

19

Type

Regular issue (Original Article)

Type Code

111

Publication Type

Journal

Publication Title

Egyptian Journal of Botany

Publication Link

https://ejbo.journals.ekb.eg/

MainTitle

Effect of Abscisic acid on Legume Growth Rate, Seed Development and Protein Profile in <i>Pisum sativum</i> L.

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Type

Article

Created At

30 Dec 2024