This work aims at enhancing soybean ability to ameliorate soybean growth and fatty acid composition of oil yield when grown under salinity by seed priming in AsA. Treatment of 10-day-old soybean seedlings with NaCl at 100 or 200 mM for 11 days significantly lowered K⁺, K⁺/Na⁺ ratio and ascorbate (AsA) and inhibited the activities of catalase (CAT) and ascorbate peroxidase (APX), contrarily elevated Na⁺, soluble sugars, MDA and H₂O₂. Meanwhile, total fatty acids were lowered in the yielded oil of mature seeds (115 days after sowing) concurrent with decreases in unsaturated fatty acids but increased saturated fatty acids (particularly palmitic and stearic). Nonetheless, seed priming in AsA improved growth coincided with raised K⁺, K⁺/Na⁺ ratio and AsA as well as CAT and APX activities concomitantly with retractions in the accumulated Na⁺, osmolytes and oxidative stress indices. Besides, saturated fatty acids were decreased but unsaturated fatty acids were increased synchronized with rises'' in total pool. This finding suggests the alleviation of salinity by priming in AsA through enhancing osmoprotection and antioxidants in addition to improving oil quality particularly omega fatty acids.