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281379

Impact of Thermotolerance Response on the Physiological and Genomic Variation of <i>Fusarium oxysporum</i>

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Last updated: 01 Jan 2025

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Abstract

Fusarium oxysporum strain F2 was isolated from the soil. It was morphologically and molecularly identified. The isolate showed promising thermotolerance characteristics. This was shown through the production of permanent proteins after 120 minutes of pre-thermal exposure at 40 ͦ C, as detected by SDS-PAGE. On the other hand, the RAPD marker revealed higher genetic variations produced by Fusarium oxysporum than the ISSR marker. Thirteen ISSR primers yielded 137 total bands, of which 81 were polymorphic bands with a percentage of 59.12%. While 18 RAPD primers yielded 223 total bands, 141 of which were polymorphic bands with a percentage of 63.23%. Notably, elevated temperatures triggered the isolate to enhance the production of hydrolytic enzymes. Fusarium oxysporum strain F2 produced more cellulolytic and pectinolytic enzymes until 120 min pre-thermal exposure at 40 ͦ C, displaying 3.262 and 1.797 (IU/ml), with corresponding percentages of activation 82.031 % and 73.288 %, respectively. This promising yield of cellulolytic activity could be employed in biofuel production.

DOI

10.21608/ejbo.2023.143391.2015

Keywords

Cell viability, <i>Fusarium oxysporium</i>, Heat shocked Proteins (Hsps), hydrolytic enzymes, ISSR and RAPD markers, SDS- gel electrophoresis

Authors

First Name

Laila M.

Last Name

Kasem

MiddleName

-

Affiliation

Agricultural Genetic Engineering Research Institute, Agricultural Research Centre, Giza 12619, Egypt

Email

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City

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Orcid

-

First Name

H.S.H.

Last Name

Attaby

MiddleName

-

Affiliation

Botany and Microbiology Department, Faculty of Science, Cairo University, Giza, Egypt

Email

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City

-

Orcid

-

First Name

Dina. A.

Last Name

El Amir

MiddleName

-

Affiliation

Agricultural Genetic Engineering Research Institute, Agricultural Research Centre, Giza 12619, Egypt

Email

-

City

-

Orcid

-

First Name

Shafik D.

Last Name

Ibrahima

MiddleName

-

Affiliation

Agricultural Genetic Engineering Research Institute, Agricultural Research Centre, Giza 12619, Egypt

Email

-

City

-

Orcid

-

First Name

K. H.

Last Name

Radwan

MiddleName

-

Affiliation

Agricultural Genetic Engineering Research Institute, Agricultural Research Centre, Giza 12619, Egypt , National Biotechnology Network of Expertise, ASRT

Email

-

City

-

Orcid

-

First Name

Neveen. M.

Last Name

Khalil

MiddleName

-

Affiliation

Botany and Microbiology Department, Faculty of Science, Cairo University, Giza, Egypt

Email

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City

-

Orcid

-

First Name

Ahmed. E.

Last Name

Ibrahim

MiddleName

-

Affiliation

Botany and Microbiology Department, Faculty of Science, Cairo University, Giza, Egypt

Email

ahelsayed@sci.cu.edu.eg

City

Cairo

Orcid

-

Volume

63

Article Issue

2

Related Issue

41028

Issue Date

2023-05-01

Receive Date

2022-06-07

Publish Date

2023-05-01

Page Start

491

Page End

504

Print ISSN

0375-9237

Online ISSN

2357-0350

Article File

EJBO_Volume 63_Issue 2_Pages 491-504.pdf

PDF . 2.0MB

Link

https://ejbo.journals.ekb.eg/article_281379.html

Detail API

https://ejbo.journals.ekb.eg/service?article_code=281379

Order

12

Type

Regular issue (Original Article)

Type Code

111

Publication Type

Journal

Publication Title

Egyptian Journal of Botany

Publication Link

https://ejbo.journals.ekb.eg/

MainTitle

Impact of Thermotolerance Response on the Physiological and Genomic Variation of <i>Fusarium oxysporum</i>

Details

Type

Article

Created At

30 Dec 2024