Nanomagnetic materials may be especially helpful in treatment of bacterial infections. In order to prevent infection and accelerate wound healing, examples include the use of NPs in antibacterial for pharmaceutical materials. This work aimed to evaluate the antimicrobial activity of Spinel and hexaferrites magnetic nanoparticles. The nanoparticles of spinel ferrites (MgFe2O4, CoFe2O4) and hexaferrites (Ba2Co2Fe12O22, BaFe12O19) were synthesized using the solgel and coprecipitation method respectively. The prepared samples were characterized by various techniques such as X-Ray diffraction (XRD), transmission electron microscopy (TEM), and antimicrobial activity of different concentrations of MgFe2O4, CoFe2O4, Ba2Co2Fe12O22 and BaFe12O19 w tested on some microorganisms of Gram-positive (Staphylococcus aureus and Bacillus subtlus), and Gram-negative (Escherichia coli) bacteria. It was found that the spinel and hexaferrites displayed different levels of antibacterial activities against all tested microorganisms. The study reported validated the dominance of M-type (BaFe12O19) as an antibacterial agent for Gram-negative and positive bacteria over MgFe2O4, CoFe2O4, and Ba2Co2Fe12O22. The results also suggested that the created magnetic nanoparticles might be used as antibacterial agents.