Inorganic mesoporous nanomaterials are highly suitable for adsorbing and loading bioactive medicinal substances due to their unique chemical and physical properties. Recently, bismuth oxide nanoparticles have gained attention for their large surface area and biocompatibility, positioning them as promising carriers for drug delivery. In this study, amoxicillin antibiotic was loaded into mesoporous nanomaterials (Bi2O3) prepared specifically for local antibiotic delivery systems. The efficient drug-loading abilities of these nanoparticles make them highly desirable for targeted drug delivery systems. To evaluate their effectiveness in drug loading, release, and bone regeneration, we created mesoporous nanoparticles (MBiNs) using the polymer sacrificial technique in this study. Although the preparation method had minimal impact on their physicochemical properties, the nanoparticles exhibited unique microstructural features. Additional research is required to gain a comprehensive understanding of these nanoparticles and uncover their potential applications in medicine.The Bi2O3 mesoporous nanomaterials were characterized using XRD, FTIR, and TEM techniques. These prepared nanomaterials show significant potential for application in drug delivery systems for bone regeneration and in enhancing the properties of other medical products.