Our research's significance is to synthesize bioactive materials (Bioglass/ Sodium alginate) and mechanically improved them with Graphene oxide. In addition to the improvement of anti-microbial by different ratios of silver nanoparticles. Using the sol-gel method, a glass of chemical composition ( 60SiO2 - 35CaO - 5P2O5 ) was prepared , and sodium alginate polymer was added at a ratio of 1:1 to all samples named as ( BG, BG/SA, Control, 1%, 2%, 3%, and 4% ). The proportion of graphene oxide was also fixed at 0.5% of the bio-glass. The samples were examined by UV spectroscopy. The bioactivity of all samples was determined after the investigation in the simulated body fluid (SBF) as a in-vetro study . Both the pH and the concentration of some ions such as calcium and phosphorus were measured . The results showed a layer of hydroxyapatite (HA) was deposited on the surface of all samples.