The use of γ-irradiation to promote phytochemical components in numerous plants, including microalgae, has gained popularity in recent years. The aim of this investigation was to evaluate the phytochemical analysis and bioactivity evaluation ofChlorella vulgarissubjected to γ-irradiation at a dose of 200 Gy after 20 days of growth. A significant increase was recorded in the contents of phytochemical constituents of proanthocyanidins, saponins, β-carotene, vitamins (B₁, B₂, B₃, B₆, A, and K), amino acids, fatty acids, and mineral contents (Ca, Na, N, P, K, Fe, and Mg) in γ-irradiated C. vulgarisas compared to the control samples. Protein constituents typically consist of 24 standard amino acids. However, C. vulgaris had twenty essential amino acids present in sufficient concentrations. The data revealed the presence of twelve identified fatty acids. This article supports the idea of improving health perspective by incorporating C. vulgarisinto the formulation of a great number of functional foods, medicinal foods, and nutritional supplements, as it offers numerous advantages in the food industry. Therefore, using metabolites of γ-irradiated C. vulgarismay unveil its prospects as a promising drug or contribute, either directly or indirectly, to keeping living things healthy since they are natural, safe, cheap, available, and easy to obtain.