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292385

Design of Lipid nanoparticle (LNP)- RNA vaccine against Haemophilus influenza type b hcsA and hcsB genes facilitating the transport of capsular polysaccharides across the outer m

Article

Last updated: 29 Dec 2024

Subjects

-

Tags

• Dentistry, Physiotherapy, Nursing, and Pharmaceutical sciences

Abstract

Background:  Hib-related meningitis and pneumonia are severe and debilitating infectious disorders that can affect anyone. The protection provided by the Haemophilus influenzae immunisation was long-lasting. Purpose of the research: The creation of a lipid nanoparticle (LNP) RNA vaccine against the genes hcsA and hcsB that allow the movement of capsular polysaccharides over the outer membrane in Haemophilus influenzae type b. Research type: Screening experimental study. Methodology: In this study, we created an LNP-RNA vaccine for Haemophilus influenzae type b hcsA and hcsB, which are involved in the pathogen's ability to transfer capsular polysaccharides through its outer membrane. Lipid nanoparticles with a particle diameter of roughly 87 nanometers made up the vaccine delivery system. Results: In preclinical experiments conducted on animals, the vaccine's efficacy was 91%, while in Phase 1/2 clinical trials, it was only about 85%. Compared to other conventional vaccines, it had superior biological activity and fewer adverse effects. Conclusion: In our investigation, the vaccination proved a promising and effective preventative measure against bacterial illness brought on by HA.

DOI

10.21608/ijhegy.2023.199193.1009

Keywords

Haemophilus influenza, vaccine, RNA, Meningitis

Authors

First Name

Mohammed

Last Name

Kassab

MiddleName

M

Affiliation

Instructor of microbiology,immunology,faculty of pharmacy,Cairo university,Egypt Specialist of pharmacology, toxicology and clinical pharmacy.

Email

ksabmhmd676@gmail.com

City

cairo

Orcid

0000-0003-2554-0663

Volume

1

Article Issue

3

Related Issue

43604

Issue Date

2023-10-01

Receive Date

2023-03-10

Publish Date

2023-10-01

Page Start

113

Page End

121

Print ISSN

2974-3613

Online ISSN

2974-3621

Link

https://ijhegy.journals.ekb.eg/article_292385.html

Detail API

https://ijhegy.journals.ekb.eg/service?article_code=292385

Order

292,385

Type

Original Article

Type Code

2,586

Publication Type

Journal

Publication Title

International Journal of Health Sciences (Egypt)

Publication Link

https://ijhegy.journals.ekb.eg/

MainTitle

Design of Lipid nanoparticle (LNP)- RNA vaccine against Haemophilus influenza type b hcsA and hcsB genes facilitating the transport of capsular polysaccharides across the outer membrane

Details

Type

Article

Created At

29 Dec 2024