Aim: To counteract Hepatitis C viral lethality, an insect recombinant DNA vaccine of Hepatitis C virus genotype 4 subtype 4a was developed using E1, E2, and Capsid protein structural proteins. Methodology: In the current study, HCV-like particles vaccinations composed of coherent structural proteins (E1, E2, and Capsid protein) of HCV were created in an expression host insect cell line employing a Baculovirus expression vector. Recombinant Baculovirus VLPs were produced by modified bac-to-bac site-specific transposition and purified via affinity chromatography. The current vaccine's immunogenicity was assessed in preclinical animal testing on transgenic mice, followed by clinical trials stages 1/2. Results: The current vaccine achieved 71% efficacy in animal models and 63% efficacy throughout stages 1-2 of human clinical trials. They have little biological influence and have few negative implications. The effect lingered for a time. An HCV-like particle immunization improved both humoral and cell-mediated protection against hepatitis C virus infection. Advantages: It is not feasible to revert to virulence.Cons: Because the vaccine virus cannot be expelled or transferred to people who have not received the immunization, it does not contribute to herd immunity against this viral infection. Conclusions: The immunization proved effective in avoiding HCV virus infection in the current study. To counteract the HCV virus's rapid mutation rate, it must be updated on a frequent basis.