Microalgae culturing with fungal hyphae has attracted extensive consideration. This study examined the beneficial interaction between microalgae and fungi under artificial continuous illumination with fluorescent lamps set to 60 μEm−1 s−1 intensity at room temperature. The research will evaluate the algae growth dynamics at different co-cultivation times with different algal fungal loads. Highlight the species-specific dependence in terms of chlorophyll a content and biomass production. The studied microalgae encompass various characteristics, including freshwater and marine origins, motile, and non-motile properties namely C.vulgaris, N.oculata, and T.chuii and filamentous fungi A.fumigatus, R.arrhizus, and M.alpina. The growth of the symbiotic system within 72 hrs. indicated that the maximum biomass of 619.2 ± 0.97 mg/L was achieved between C.vulgaris and A.fumigatus in a ratio of 1:1. Consortia of T.chuii and A.fumigatus in a ratio of 2:1 recorded the highest chlorophyll a content of 8.16 ± 0.11 mg/L. Observing the structures of co-cultivated organisms under the light microscope validated that the microalgae cells and fungal mycelia were mutually tangled together to form the network morphology.